| Application | SIGMA FAST™ BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium) Tablets have been developed for use in immunochemistry as a precipitating substrate for the detection of alkaline phosphatase activity. Common uses are in immunoblotting or dot blotting. It is less commonly used in immunohistochemistry. These tablets require no additional buffers or steps to prepare an active substrate solution. |
| Reconstitution | Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5. |
| Legal Information | BCIP is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| | SIGMAFAST is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
